Abstract Background Recently, various studies have revealed that 3D cell spheroids have several advantages over 2D cells in stem cell culture.However, conventional 3D spheroid culture methods have some disadvantages and limitations such as time required for spheroid formation and complexity of the experimental process.Here, we used acoustic levitat
Abstract Methadone Maintenance Treatment (MMT) is among the most widely studied treatments for opiate dependence with proven benefits for patients and society.When misused, however, methadone can also be lethal.The issue of methadone diversion is a major concern for all MMT programs.A potential source for such diversion is from those MMT patients w
Helicobacter pylori, a spiral-shaped Gram-negative bacterium, has been classified as a class I carcinogen by the World Health Organization and recognized as the causative agent for peptic ulcers, duodenal ulcer, gastritis, mucosa-associated lymphoid tissue lymphomas, and gastric cancer.Owing to their alarming rate of drug resistance, eradication of
Calcium is an essential element in our body and plays a vital role in moderating calcium signalling.Calcium is also called the second messenger.Calcium signalling depends on cytosolic calcium concentration.In this study, we focus on cellular calcium fluctuations with different buffers, including calcium-binding buffers, using the Bundle Hilfer frac